Regulatory

Part:BBa_K656000

Designed by: Evan Clark   Group: iGEM11_Brown-Stanford   (2011-05-03)


LexA Promoter

This is the promoter for the LexA gene. LexA is a repressor protein that prevents transcription of the SOS repair pathway unless DNA damage is detected by binding to the SOS genes' promoters and inactivating them. In the presence of single stranded DNA due to DNA damage, another protein, RecA, interacts with ss-DNA. The RecA-ssDNA complex then interacts with LexA, causing it to cleave off the SOS gene promoter, allowing transcription to go forward.

LexA regulates its own promoter

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 9
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None